CD169重组兔单克隆抗体 ,1mg/ml

中文名称：CD169重组兔单克隆抗体
别    名：CD 169; CD-169; dJ1009E24.1; DKFZp667F058; FLJ00051; FLJ00055; FLJ00073; FLJ00411; FLJ32150; Sialic acid binding Ig like lectin 1; Sialic acid binding immunoglobulin like lectin 1; Siglec 1; SN; sialoadhesin; SN_HUMAN. 
研究领域免疫学：  微生物学  b-淋巴细胞 
抗体来源：Rabbit
克隆类型：Monoclonal
克 隆 号：B4H12
交叉反应： Human,
产品应用：WB=1:200-1000 IHC-P=1:200-500 Flow-Cyt=1:50-100 IF=1:50 （石蜡切片需做抗原修复）
not yet tested in other applications.
optimal dilutions/concentrations should be determined by the end user.
理论分子量：186kDa
细胞定位细胞膜 ：分泌型蛋白
性    状：Liquid
浓    度：1mg/ml
免 疫 原：KLH conjugated synthetic peptide derived from human SIGLEC1
亚    型：IgG
纯化方法：affinity purified by Protein A
缓 冲 液：0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
保存条件：Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
注意事项：This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.
PubMedPubMed
产品介绍： This gene encodes a member of the immunoglobulin superfamily. The encoded protein is a lectin-like adhesion molecule that binds glycoconjugate ligands on cell surfaces in a sialic acid-dependent manner. It is a type I transmembrane protein expressed only by a subpopulation of macrophages and is involved in mediating cell-cell interactions. Alternative splicing produces a transcript variant encoding an isoform that is soluble rather than membrane-bound; however, the full-length nature of this variant has not been determined. [provided by RefSeq, Jul 2008].

Function:
Acts as an endocytic receptor mediating clathrin dependent endocytosis. Macrophage-restricted adhesion molecule that mediates sialic-acid dependent binding to lymphocytes, including granulocytes, monocytes, natural killer cells, B-cells and CD8 T-cells. Preferentially binds to alpha-2,3-linked sialic acid. Binds to SPN/CD43 on T-cells. May play a role in hemopoiesis.

Subunit:
Isoform 1: Cell membrane; Single-pass type I membrane protein. Isoform 2: Secreted.

Subcellular Location:
Expressed by macrophages in various tissues. High levels are found in spleen, lymph node, perivascular macrophages in brain and lower levels in bone marrow, liver Kupffer cells and lamina propria of colon and lung. Also expressed by inflammatory macrophages in rheumatoid arthritis.

Similarity:
Belongs to the immunoglobulin superfamily. SIGLEC (sialic acid binding Ig-like lectin) family.
Contains 16 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.

SWISS:
Q9BZZ2

Gene ID:
6614
产品图片
CD169，又称sialoadhesin（Sn）或Siglee-1，这是一种200kDa的I型跨膜糖蛋白，由炎性巨噬细胞特异性表达，在细胞-细胞或细胞-基质的相互作用中发挥主要作用。可识别乳癌细胞的MUC1（CD227）和T细胞的CD43。CD169在脾、淋巴结、骨髓的基质巨噬细胞上表达最强，在肝、肠、肺的长驻巨噬细胞上中等表达，慢性炎组织的巨噬细胞，例如类风湿性滑膜、动脉粥样硬化性斑块，也强烈表达CD169。

Tissue: Human liver 
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure 
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000 
Primary incubation condition: 1 hour at room temperature 
Counter stain: Hematoxylin 
Comment: Color brown is the positive signal for

Tissue: Human colon 
Section type: Formalin fixed & Paraffin -embedded section
Retrieval method: High temperature and high pressure 
Retrieval buffer: Tris/EDTA buffer, pH 9.0 Primary ab dilution: 1:1000 
Primary ab incubation condition: 1 hour at room temperature
Counter stain: Hematoxylin 
Comment: Color brown is the positive signal for